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ti2 e eclipse inverted light microscope  (Nikon)


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    Structured Review

    Nikon ti2 e eclipse inverted light microscope
    Ti2 E Eclipse Inverted Light Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ti2+e+eclipse+inverted+light+microscope/Objectives/pm41713129-113-18-17
    Average 99 stars, based on 59633 article reviews
    ti2 e eclipse inverted light microscope - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: PMCA Ca 2+ clearance in dental enamel cells depends on the magnitude of cytosolic Ca 2+
    Article Snippet: All reagents were obtained from Sigma-Aldrich, USA. c Ca 2+ transients of secretory and maturation cells were measured at room (24 ± 2°C) temperature using a polycarbonate 260 μl chamber (Harvard Bioscience Inc., USA; #:RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). .. Cells were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 5–6 ml per minute with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc., USA).

    Article Title: Elevated mitochondrial metabolism in Down syndrome iPSCs reduces commitment to neuroectoderm
    Article Snippet: The identity of MCU was confirmed by the addition of 10 μM of the MCU inhibitor Ruthenium Red (RuRed) Ca 2+ transients were measured at room temperature (20-22°C) using a polycarbonate 260 μL chamber (Harvard Bioscience Inc., USA cat #RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). iPSC cells were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 4-5 ml per min with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc., USA). ..

    Article Title: Na+/Ca2+ exchange in enamel cells is dominated by the K+-dependent NCKX exchanger.
    Article Snippet: Calcium (Ca2+) extrusion is an essential function of the enamel-forming ameloblasts, providing Ca2+ for extracellular mineralization.. The plasma membrane Ca2+ ATPases (PMCAs) remove cytosolic Ca2+ (cCa) and were recently shown to be efficient when ameloblasts experienced low cCa elevation.. Sodium–calcium (Na+/Ca2+) exchange has higher capacity to extrude cCa, but there is limited evidence on the function of the two main families of Na+/Ca2+ exchangers in enamel formation.

    Article Title: Age-Related Decline in NCKX4-Mediated Calcium Clearance Accelerates Aortic Remodeling and Drives Early Vascular Aging
    Article Snippet: Cytosolic Ca 2+ transients were measured at room temperature (24 ± 2°C) using a polycarbonate 260 μl chamber (cat #RC21BR; Harvard Bioscience Inc.) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon) controlled by computer software (NIS Elements version 5.20.01). .. VSMCs were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 5-6 ml per minute with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc.).

    Article Title: Overexpression of RCAN1 , a Gene on Human Chromosome 21 , Alters Cell Redox and Mitochondrial Function in Enamel Cells.
    Article Snippet: Cells were perfused with ATP (100 μM) (Abcam) in a nominal Ca2+ solution 60 sec after starting the recordings, and then both ATP and Ca2+ were removed 120 s after starting the recordings. .. Fluorescence recordings were obtained using a Nikon Ti2-E Eclipse inverted light microscope equipped with a digital SLR camera controlled by a computer software (NIS-Elements, USA). ..

    Article Title: <scp>PMCA</scp> Ca <sup>2+</sup> clearance in dental enamel cells depends on the magnitude of cytosolic Ca <sup>2+</sup>
    Article Snippet: All reagents were obtained from Sigma- Aldrich, USA. cCa 2+ transients of secretory and maturation cells were measured at room (24 ± 2°C) temperature using a polycarbonate 260 μl chamber (Harvard Bioscience Inc., USA; #:RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2- E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS- Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). .. Cells were continuously perfused by a six- way perfusion system (VC- 8 valve controller) at 5– 6 ml per minute with a common outlet 0.28- mm tube driven by controlled valves (Harvard Bioscience Inc., USA).

    Light Microscopy:

    Article Title: PMCA Ca 2+ clearance in dental enamel cells depends on the magnitude of cytosolic Ca 2+
    Article Snippet: All reagents were obtained from Sigma-Aldrich, USA. c Ca 2+ transients of secretory and maturation cells were measured at room (24 ± 2°C) temperature using a polycarbonate 260 μl chamber (Harvard Bioscience Inc., USA; #:RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). .. Cells were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 5–6 ml per minute with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc., USA).

    Article Title: NCKX4 regulates hippocampal Ca 2+ homeostasis and contributes to contextual memory and anxiety-related behaviours.
    Article Snippet: Briefly, cells were incubated for 30–35 min at room temperature with 1 μM of the ratiometric Ca2+ probe Fura-2-AM (Thermo Fisher, USA) using an extracellular solution composed of (in mM): 155 NaCl; 4.5 KCl; 2.5 CaCl2; 1.0 MgCl2; 10 D-glucose; and 10 HEPES (Sigma-Aldrich, USA), pH 7.4. .. Coverslips were attached to a polycarbonate 260 μl chamber (cat #RC21BR; Harvard Bioscience Inc.) mounted on a Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon) controlled by computer software (NIS Elements version 5.20.01). .. Briefly, cells were incubated for 30–35 min at room temperature with 1 μM of the ratiometric Ca2+ probe Fura-2-AM (Thermo Fisher, USA) using an extracellular solution composed of (in mM): 155 NaCl; 4.5 KCl; 2.5 CaCl2; 1.0 MgCl2; 10 D-glucose; and 10 HEPES (Sigma-Aldrich, USA), pH 7.4.

    Article Title: Elevated mitochondrial metabolism in Down syndrome iPSCs reduces commitment to neuroectoderm
    Article Snippet: The identity of MCU was confirmed by the addition of 10 μM of the MCU inhibitor Ruthenium Red (RuRed) Ca 2+ transients were measured at room temperature (20-22°C) using a polycarbonate 260 μL chamber (Harvard Bioscience Inc., USA cat #RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). iPSC cells were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 4-5 ml per min with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc., USA). ..

    Article Title: Age-Related Decline in NCKX4-Mediated Calcium Clearance Accelerates Aortic Remodeling and Drives Early Vascular Aging
    Article Snippet: Cytosolic Ca 2+ transients were measured at room temperature (24 ± 2°C) using a polycarbonate 260 μl chamber (cat #RC21BR; Harvard Bioscience Inc.) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon) controlled by computer software (NIS Elements version 5.20.01). .. VSMCs were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 5-6 ml per minute with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc.).

    Article Title: Overexpression of RCAN1 , a Gene on Human Chromosome 21 , Alters Cell Redox and Mitochondrial Function in Enamel Cells.
    Article Snippet: Cells were perfused with ATP (100 μM) (Abcam) in a nominal Ca2+ solution 60 sec after starting the recordings, and then both ATP and Ca2+ were removed 120 s after starting the recordings. .. Fluorescence recordings were obtained using a Nikon Ti2-E Eclipse inverted light microscope equipped with a digital SLR camera controlled by a computer software (NIS-Elements, USA). ..

    Article Title: <scp>PMCA</scp> Ca <sup>2+</sup> clearance in dental enamel cells depends on the magnitude of cytosolic Ca <sup>2+</sup>
    Article Snippet: All reagents were obtained from Sigma- Aldrich, USA. cCa 2+ transients of secretory and maturation cells were measured at room (24 ± 2°C) temperature using a polycarbonate 260 μl chamber (Harvard Bioscience Inc., USA; #:RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2- E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS- Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). .. Cells were continuously perfused by a six- way perfusion system (VC- 8 valve controller) at 5– 6 ml per minute with a common outlet 0.28- mm tube driven by controlled valves (Harvard Bioscience Inc., USA).

    Software:

    Article Title: PMCA Ca 2+ clearance in dental enamel cells depends on the magnitude of cytosolic Ca 2+
    Article Snippet: All reagents were obtained from Sigma-Aldrich, USA. c Ca 2+ transients of secretory and maturation cells were measured at room (24 ± 2°C) temperature using a polycarbonate 260 μl chamber (Harvard Bioscience Inc., USA; #:RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). .. Cells were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 5–6 ml per minute with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc., USA).

    Article Title: NCKX4 regulates hippocampal Ca 2+ homeostasis and contributes to contextual memory and anxiety-related behaviours.
    Article Snippet: Briefly, cells were incubated for 30–35 min at room temperature with 1 μM of the ratiometric Ca2+ probe Fura-2-AM (Thermo Fisher, USA) using an extracellular solution composed of (in mM): 155 NaCl; 4.5 KCl; 2.5 CaCl2; 1.0 MgCl2; 10 D-glucose; and 10 HEPES (Sigma-Aldrich, USA), pH 7.4. .. Coverslips were attached to a polycarbonate 260 μl chamber (cat #RC21BR; Harvard Bioscience Inc.) mounted on a Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon) controlled by computer software (NIS Elements version 5.20.01). .. Briefly, cells were incubated for 30–35 min at room temperature with 1 μM of the ratiometric Ca2+ probe Fura-2-AM (Thermo Fisher, USA) using an extracellular solution composed of (in mM): 155 NaCl; 4.5 KCl; 2.5 CaCl2; 1.0 MgCl2; 10 D-glucose; and 10 HEPES (Sigma-Aldrich, USA), pH 7.4.

    Article Title: Elevated mitochondrial metabolism in Down syndrome iPSCs reduces commitment to neuroectoderm
    Article Snippet: The identity of MCU was confirmed by the addition of 10 μM of the MCU inhibitor Ruthenium Red (RuRed) Ca 2+ transients were measured at room temperature (20-22°C) using a polycarbonate 260 μL chamber (Harvard Bioscience Inc., USA cat #RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). iPSC cells were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 4-5 ml per min with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc., USA). ..

    Article Title: Age-Related Decline in NCKX4-Mediated Calcium Clearance Accelerates Aortic Remodeling and Drives Early Vascular Aging
    Article Snippet: Cytosolic Ca 2+ transients were measured at room temperature (24 ± 2°C) using a polycarbonate 260 μl chamber (cat #RC21BR; Harvard Bioscience Inc.) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2-E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS-Qi2; Nikon) controlled by computer software (NIS Elements version 5.20.01). .. VSMCs were continuously perfused by a six-way perfusion system (VC-8 valve controller) at 5-6 ml per minute with a common outlet 0.28-mm tube driven by controlled valves (Harvard Bioscience Inc.).

    Article Title: Overexpression of RCAN1 , a Gene on Human Chromosome 21 , Alters Cell Redox and Mitochondrial Function in Enamel Cells.
    Article Snippet: Cells were perfused with ATP (100 μM) (Abcam) in a nominal Ca2+ solution 60 sec after starting the recordings, and then both ATP and Ca2+ were removed 120 s after starting the recordings. .. Fluorescence recordings were obtained using a Nikon Ti2-E Eclipse inverted light microscope equipped with a digital SLR camera controlled by a computer software (NIS-Elements, USA). ..

    Article Title: <scp>PMCA</scp> Ca <sup>2+</sup> clearance in dental enamel cells depends on the magnitude of cytosolic Ca <sup>2+</sup>
    Article Snippet: All reagents were obtained from Sigma- Aldrich, USA. cCa 2+ transients of secretory and maturation cells were measured at room (24 ± 2°C) temperature using a polycarbonate 260 μl chamber (Harvard Bioscience Inc., USA; #:RC21BR) mounted on an inverted microscope coupled to a perfusion system electrically controlled. .. Fluorescence recordings were obtained using the Nikon Ti2- E Eclipse inverted light microscope, equipped with an objective (Nikon S Fluor × 20; numerical aperture: 0.75) and a digital SLR camera (DS- Qi2; Nikon, Japan) controlled by computer software (NIS Elements version 5.20.01, USA). .. Cells were continuously perfused by a six- way perfusion system (VC- 8 valve controller) at 5– 6 ml per minute with a common outlet 0.28- mm tube driven by controlled valves (Harvard Bioscience Inc., USA).



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